The sample is then diluted in 10 fold serial dilutions and plated in an appropriate medium.įollowing incubation, the number of colonies present in the plate is counted. In this method, the substance to be tested if not in liquid form is ground and dissolved in a suitable liquid medium. To respect the necessary “short” time interval between agar inoculation and spreading.To apply a balanced spread technique using a glass spreader to spread the inoculum evenly on the agar surface.To make accurate dilutions using pipettes (master serial dilution technique).

To get optimum result from spread plate technique students must be careful

Spread plate technique is a viable counting method employed to plate a liquid sample for the purpose of isolating or counting the bacteria present in that sample. A perfect spread plate technique will result in visible and isolated colonies of bacteria that are evenly distributed in the plate and are countable. The technique is most commonly applied for microbial testing of foods or any other samples or to isolate and identify a variety of microbial flora present in the environmental samples e.g.

Serial dilution lab conclusion